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eo771 crl 3461 tumor cell lines  (ATCC)


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    ATCC eo771 crl 3461 tumor cell lines
    ( A ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation and vaccination (mRNA, 15 μg per mouse). ( B ) Photographs of the lungs. ( C ) H&E staining images of the lung tissues. Scale bars, 3000 μm. ( D ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the spleens. ( E ) Flow cytometry analysis of the population of IFN-γ + CD8 + CD3 + T cells in the spleens. ( F ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the blood. ( G ) Flow cytometry analysis of the population CD62L low CD44 high T cells (gated on CD8 + CD3 + cells) in the spleens. ( H ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation, vaccination (mRNA, 15 μg per mouse), and tumor rechallenge. sc, subcutaneous. ( I ) Photographs of the tumors. ( J ) Individual tumor growth curves (PBS, gray; ThrCo LNP, steel blue). TFS, tumor-free survival. ( K ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( L ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( M ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in <t>EO771</t> tumors. ( N ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. Data are presented as means ± SD from n biologically independent samples [(D) to (G), (I), and (K) to (N), n = 3]; [(J), n = 5]. Statistical significance was analyzed by one-way ANOVA with Tukey’s multiple comparisons test for [(D) to (G)] and t test for [(K) to (N)].
    Eo771 Crl 3461 Tumor Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eo771 crl 3461 tumor cell lines/product/ATCC
    Average 98 stars, based on 222 article reviews
    eo771 crl 3461 tumor cell lines - by Bioz Stars, 2026-03
    98/100 stars

    Images

    1) Product Images from "Replacing cholesterol and PEGylated lipids with zwitterionic ionizable lipids in LNPs for spleen-specific mRNA translation"

    Article Title: Replacing cholesterol and PEGylated lipids with zwitterionic ionizable lipids in LNPs for spleen-specific mRNA translation

    Journal: Science Advances

    doi: 10.1126/sciadv.ady6460

    ( A ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation and vaccination (mRNA, 15 μg per mouse). ( B ) Photographs of the lungs. ( C ) H&E staining images of the lung tissues. Scale bars, 3000 μm. ( D ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the spleens. ( E ) Flow cytometry analysis of the population of IFN-γ + CD8 + CD3 + T cells in the spleens. ( F ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the blood. ( G ) Flow cytometry analysis of the population CD62L low CD44 high T cells (gated on CD8 + CD3 + cells) in the spleens. ( H ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation, vaccination (mRNA, 15 μg per mouse), and tumor rechallenge. sc, subcutaneous. ( I ) Photographs of the tumors. ( J ) Individual tumor growth curves (PBS, gray; ThrCo LNP, steel blue). TFS, tumor-free survival. ( K ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( L ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( M ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. ( N ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. Data are presented as means ± SD from n biologically independent samples [(D) to (G), (I), and (K) to (N), n = 3]; [(J), n = 5]. Statistical significance was analyzed by one-way ANOVA with Tukey’s multiple comparisons test for [(D) to (G)] and t test for [(K) to (N)].
    Figure Legend Snippet: ( A ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation and vaccination (mRNA, 15 μg per mouse). ( B ) Photographs of the lungs. ( C ) H&E staining images of the lung tissues. Scale bars, 3000 μm. ( D ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the spleens. ( E ) Flow cytometry analysis of the population of IFN-γ + CD8 + CD3 + T cells in the spleens. ( F ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the blood. ( G ) Flow cytometry analysis of the population CD62L low CD44 high T cells (gated on CD8 + CD3 + cells) in the spleens. ( H ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation, vaccination (mRNA, 15 μg per mouse), and tumor rechallenge. sc, subcutaneous. ( I ) Photographs of the tumors. ( J ) Individual tumor growth curves (PBS, gray; ThrCo LNP, steel blue). TFS, tumor-free survival. ( K ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( L ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( M ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. ( N ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. Data are presented as means ± SD from n biologically independent samples [(D) to (G), (I), and (K) to (N), n = 3]; [(J), n = 5]. Statistical significance was analyzed by one-way ANOVA with Tukey’s multiple comparisons test for [(D) to (G)] and t test for [(K) to (N)].

    Techniques Used: Staining, Flow Cytometry, Binding Assay



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    eo771 crl 3461 tumor cell lines  (ATCC)
    98
    ATCC eo771 crl 3461 tumor cell lines
    ( A ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation and vaccination (mRNA, 15 μg per mouse). ( B ) Photographs of the lungs. ( C ) H&E staining images of the lung tissues. Scale bars, 3000 μm. ( D ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the spleens. ( E ) Flow cytometry analysis of the population of IFN-γ + CD8 + CD3 + T cells in the spleens. ( F ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the blood. ( G ) Flow cytometry analysis of the population CD62L low CD44 high T cells (gated on CD8 + CD3 + cells) in the spleens. ( H ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation, vaccination (mRNA, 15 μg per mouse), and tumor rechallenge. sc, subcutaneous. ( I ) Photographs of the tumors. ( J ) Individual tumor growth curves (PBS, gray; ThrCo LNP, steel blue). TFS, tumor-free survival. ( K ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( L ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( M ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in <t>EO771</t> tumors. ( N ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. Data are presented as means ± SD from n biologically independent samples [(D) to (G), (I), and (K) to (N), n = 3]; [(J), n = 5]. Statistical significance was analyzed by one-way ANOVA with Tukey’s multiple comparisons test for [(D) to (G)] and t test for [(K) to (N)].
    Eo771 Crl 3461 Tumor Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eo771 crl 3461 tumor cell lines/product/ATCC
    Average 98 stars, based on 1 article reviews
    eo771 crl 3461 tumor cell lines - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation and vaccination (mRNA, 15 μg per mouse). ( B ) Photographs of the lungs. ( C ) H&E staining images of the lung tissues. Scale bars, 3000 μm. ( D ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the spleens. ( E ) Flow cytometry analysis of the population of IFN-γ + CD8 + CD3 + T cells in the spleens. ( F ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the blood. ( G ) Flow cytometry analysis of the population CD62L low CD44 high T cells (gated on CD8 + CD3 + cells) in the spleens. ( H ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation, vaccination (mRNA, 15 μg per mouse), and tumor rechallenge. sc, subcutaneous. ( I ) Photographs of the tumors. ( J ) Individual tumor growth curves (PBS, gray; ThrCo LNP, steel blue). TFS, tumor-free survival. ( K ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( L ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( M ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. ( N ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. Data are presented as means ± SD from n biologically independent samples [(D) to (G), (I), and (K) to (N), n = 3]; [(J), n = 5]. Statistical significance was analyzed by one-way ANOVA with Tukey’s multiple comparisons test for [(D) to (G)] and t test for [(K) to (N)].

    Journal: Science Advances

    Article Title: Replacing cholesterol and PEGylated lipids with zwitterionic ionizable lipids in LNPs for spleen-specific mRNA translation

    doi: 10.1126/sciadv.ady6460

    Figure Lengend Snippet: ( A ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation and vaccination (mRNA, 15 μg per mouse). ( B ) Photographs of the lungs. ( C ) H&E staining images of the lung tissues. Scale bars, 3000 μm. ( D ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the spleens. ( E ) Flow cytometry analysis of the population of IFN-γ + CD8 + CD3 + T cells in the spleens. ( F ) Flow cytometry analysis of the population of CD8 + CD3 + T cells that bear T cell receptors binding to H2Kb OVA tetramer-SIINFEKL in the blood. ( G ) Flow cytometry analysis of the population CD62L low CD44 high T cells (gated on CD8 + CD3 + cells) in the spleens. ( H ) Schematic illustration of the timeline for B16F10-OVA tumor inoculation, vaccination (mRNA, 15 μg per mouse), and tumor rechallenge. sc, subcutaneous. ( I ) Photographs of the tumors. ( J ) Individual tumor growth curves (PBS, gray; ThrCo LNP, steel blue). TFS, tumor-free survival. ( K ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( L ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in B16F10-OVA tumors. ( M ) Flow cytometry analysis of the population of CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. ( N ) Flow cytometry analysis of the population of IFN-γ + CD8 + T cells (gated on CD3 + CD45 + cells) in EO771 tumors. Data are presented as means ± SD from n biologically independent samples [(D) to (G), (I), and (K) to (N), n = 3]; [(J), n = 5]. Statistical significance was analyzed by one-way ANOVA with Tukey’s multiple comparisons test for [(D) to (G)] and t test for [(K) to (N)].

    Article Snippet: DC2.4 cell line (SCC142) was purchased from Sigma-Aldrich, and B16F10-OVA (CRL-6475) and EO771 (CRL-3461) tumor cell lines were purchased from American Type Culture Collection.

    Techniques: Staining, Flow Cytometry, Binding Assay